Tissue culture is a genetic engineering method in which a plant cell or tissue is isolated and placed in a suitable medium to produce a complete plant through using nutrients and growing in the culture medium. Plant tissue culture proposed by Haberlint at the German Academy of Sciences in 1902 and was called Invitro, Laboratory Culture and Sterile Culture. Due to the many benefits of tissue culture, its use in agriculture has grown significantly recently. Advantages of tissue culture include the rapid proliferation of clones, genetic uniformity, selection of plants with special characteristics, preservation of the mother plant in optimal conditions, control of physical conditions of culture and growth environment, preservation of germplasm of different plants, production of incompatible hybrids through embryo culture, the production of haploid plants, the permanent production of plants in all seasons, the propagation of hardy plants.

Aloe Vera: This plant is very important economically and medicinally and due to its very slow natural and traditional reproduction, it cannot meet the needs of the country’s pharmaceutical industry. Therefore, the micropropagation method is used for the rapid propagation of the Aloe plant.

Tea: Micropropagation of this plant and production of young seedlings is very necessary to replace the old plants in Iranian tea gardens more quickly.

Paulownia: Micropropagation of this plant is an essential factor for the forestry of Paulownia clones and has many benefits in seedling production.

Physalis: The exceptional medicinal properties of the fruit of this plant have caused it to be mass-produced and propagated. Therefore, micropropagation of the Physalis plant, which has valuable fruits and beneficial properties for humans, is necessary.

Achievement:

Successful micropropagation and transfer to greenhouse