Ligninolytic enzyme production from fungi

Ligninolytic enzyme production from fungi

The use of enzymes is proposed as a new method in the biological purification of environmental pollutants. In today’s world, environmental pollution is one of the most critical issues that are inevitable due to the increasing industrial life of human societies. With one-third of the world’s energy consumption coming from oil, one of the most critical environmental pollutants in surface water, groundwater and soil are heavy oil compounds, including saturated hydrocarbons. Polycyclic aromatic hydrocarbons (PAHs) are asphaltene and resin. There are several methods to remove this type of environmental pollution, one of which is biocatalysts. Biocatalysts are a set of enzymes and biological compounds that have received much attention due to their excellent environmental stability, controllability, reused capability, and wide range of substrates. This project aims to use ligninolytic enzymes to degrade petroleum compounds from the environment. These enzymes were extracted from white-rot fungi, and chromatographic methods performed purification. Purified ligninolytic enzymes will be evaluated for pH, temperature and synthetic parameters. To quantify the expression of enzymes, the measurement of enzyme activity with appropriate substrates at the proper temperature and wavelength for each of the enzymes will be measured. The activity of enzymes will be measured in the presence of target compounds (petroleum compounds). Finally, the decomposition of contaminants will be measured using chromatographic and SARA methods.

Achievement:
– Production of ligninolytic enzyme
– Cleaning up environmental pollution

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